Faculty: Yufeng Tong, PhD
Assistant Professor, Pharmacology & Toxicology
SGC Fellow, Team Leader
General Research Area: Ubiquitin Biology and Cell Signalling
The Ubiquitin Biology group at SGC Toronto focuses on understanding the structure, function, specificity, and enzymatic mechanism of E3 ubiquitin ligases and deubiquitinases(DUBs), including key enzymes involved in histone (de-)ubiquitylation and sumoylation. Particularly we are interested in the substrate recognition domains of Ring-inBetween-Ring type, HECT-type E3 ligases, and ubiquitin-specific proteases besides their catalytic domains. These families of proteins have been directly implicated in many human diseases like autism, cancer diabetes, leukemia, allergy, and etc. We are also interested in some guanine nucleotide exchange factors (GEFs) that share common domains ubiquitin ligases or DUBs, in particular the beta-propeller fold domains that are known to modulate protein-protein interaction.
We utilize the SGC’s established high-throughput structural biology platform for protein production, structure determination and biochemical assays to probe the function of these enzymes. We also collaborate with internal and external groups to identify small molecule compounds and biomolecules (ubiquitin variants) that will alter the activity of disease-related ligases and proteases.
The substrate binding domains of human SIAH E3 ubiquitin ligases are now crystal clear. Biochimica et Biophysica Acta (BBA)-General Subjects, 2016, 1861 (1):3095-3105
Structural basis of the recruitment of ubiquitin-specific protease USP15 by spliceosome recycling factor SART3. Journal of Biological Chemistry, 2016, 291 (33):17283-17292
System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes. Molecular Cell, 2016, 62(1):121-136
New insights into the RNA-binding and E3 ubiquitin ligase activities of Roquins: Scientific Reports, 2015, 5:15660
Phosphorylation-independent dual-site binding of the FHA domain of KIF13 mediates phosphoinositide transport via centaurin alpha1. Proc Natl Acad Sci U S A. 2010, 107(47):20346-20351
Structure and function of the intracellular region of the plexin-b1 transmembrane receptor. J Biol Chem. 2009, 284(51):35962-35972
For full list of publication visit: